ABSTRACT
In the present study, we report on the occurrence of paramphistomes, Fischoederius cobboldi and Paramphistomum epiclitum, in Lao PDR with the basis of molecular data. Parasite materials were collected from bovines bred in Ban Lahanam area, Savannakhet Province, Lao PDR at Lahanam public market. Morphological observations indicated 2 different species of paramphistomes. The mitochondrial gene cox1 of the specimens was successfully amplified by PCR and DNA sequencing was carried out for diagnosis of 11 specimens. Pairwise alignment of cox1 sequences were performed and confirmed F. cobboldi and P. epiclitum infecting bovines in Laos. Although there were many limiting points, as the small number of worm samples, and the restricted access of the animal host materials, we confirmed for the first time that 2 species of paramphistomes, F. cobboldi and P. epiclitum, are distributed in Lao PDR. More studies are needed to confirm the paramphistome species present in Savannakhet and its hosts to clear the natural history of these parasites of ruminants in the region and measure the impact of this parasite infection in the life and health of the local people.
Subject(s)
Animals , Cattle , Diagnosis , Genes, Mitochondrial , Helminths , Laos , Natural History , Paramphistomatidae , Parasites , Polymerase Chain Reaction , Rumen , Ruminants , Sequence Analysis, DNAABSTRACT
The surface morphology of specimens from ten different genera of amphistomes (Trematoda, Cladorchiidae) from Amazonian fishes is described and illustrated. The importance of body shape as a generic character is considered. Morphological changes as a result of growth are shown and explained in relation to the species Dadaytrema oxycephala. Additionally, Doradamphistoma bacuensis gen. et sp. n. is described from the catfish, Megalodoras irwini Eigenmann, 1925.The new genus and species is elongate and flattened, with external pharyngeal pouches, an esophageal bulb, a spherical cirrus sac, a post-bifurcal genital pore and pre-equatorial testes.
A morfologia superficial de espécimens representando dez gêneros diferentes de anfístomos (Trematoda, Cladorchiidae) de peixes amazônicos é descrita e ilustrada. A importância da forma do corpo como caracter genérico é considerada. Modificações morfológicas, como resultado do crescimento, são mostradas e explicadas em relação à espécie Dadaytrema oxycephala. Adicionalmente, Doradamphistoma bacuensis gen. et sp. n. é descrito de um bagre amazônico, Megalodoras irwini. A espécie nova é alongada e achatada, com sacos faringiais externos, um bulbo esofageano, uma bolsa de cirro esférica, um poro genital pós-bifurcal e testículos pré-equatoriais.
ABSTRACT
Two new genera and species of Paramphistomidae (Tremadota, Digenea) are described from freshwater fish of the Brazilian Amazon. Anavilhanatrema robustagen. et sp. nov. is characterized as having a cylindrical body with an anterior expansion, small suckers, a small cirrus sac, large lobate testes, a lobate ovary, long ceca and extensive vitellaria. Pacudistoma turgidagen. et sp. nov., on the other hand, is distinguished by having a stout, cylindrical body, a large oral sucker with internal diverticula, a large genital sucker, smaller lobate testes, a spherical ovary, long ceca and relatively extensive vitellaria. Both species appear to be host specific with A. robustafound in Pristobrycon striolatus(STEINDACHNER) while P. turgidaoccurus in Hyleus pacu(SCHOMBURCK).
Dois novos gêneros e espécies de Paramphistomidae (Trematoda, Vigenea) são descitos de peixes de água doce da Amazônia brasileira. Anavilhanatrema robusta gen. et sp. nov. e caracterizado porter um corpo cilíndrico com uma expansão anterior, ventosas pequenas, uma bolsa de cirro pequena, testículos grandes e lobados, um ovario lobado, cecos longos e glândulas vitelínicas extensivas. Pacudistoma turgida gen. et sp. nov., no entanto, se distingue porter um corpo cilíndrico, uma ventosa oral grande com divertículos internos, uma ventosa genital grande, testículos lobados que são menores em tamanho, um ovario esférico, cecos longos e glândulas vitelínicas que são relativamente extensivas. Ambas espécies parecem ser específicas acanto aos hospedeiros, sendo que A. robusta encontra-se em Pristobrycon striolatus (STEINDACHENER) e P. turgida ocorre em Myleus pacu (SCHOMBURGK).